Coding

Part:BBa_K5222007

Designed by: Maria Avtodeeva   Group: iGEM24_Sorbonne-U-Paris   (2024-10-02)


tphAa,tphAb,tphAc,tphB fusion protein adapted for chlamydomonas reinhardtii

In the article Characterization of the Terephthalate Degradation Genes of Comamonas sp. Strain E6, [1] it was shown that the enzymes encoded by the tphRICIA2IA3IBIA1I gene cluster are essential for transforming TPA into protocatechuic acid (PCA)

In fact, the essential enzymes for this transformation are TphA1,A2,A3 ( also noted sometimes as TphAa,Ab,Ac) and TphB

Here we created a fusion protein of 4 proteins tphAa,tphAb,tphAc,tpB an we made a codon optimization for chlamydomonas reinhardtii. Also this CDS is adapted for position B3-B5 in MoClo [2]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
    Illegal PstI site found at 246
    Illegal PstI site found at 2073
    Illegal PstI site found at 2106
    Illegal PstI site found at 2886
    Illegal PstI site found at 3063
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
    Illegal PstI site found at 93
    Illegal PstI site found at 246
    Illegal PstI site found at 2073
    Illegal PstI site found at 2106
    Illegal PstI site found at 2886
    Illegal PstI site found at 3063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1432
    Illegal BglII site found at 1723
    Illegal XhoI site found at 1482
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
    Illegal PstI site found at 246
    Illegal PstI site found at 2073
    Illegal PstI site found at 2106
    Illegal PstI site found at 2886
    Illegal PstI site found at 3063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal PstI site found at 246
    Illegal PstI site found at 2073
    Illegal PstI site found at 2106
    Illegal PstI site found at 2886
    Illegal PstI site found at 3063
    Illegal NgoMIV site found at 561
    Illegal NgoMIV site found at 789
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 2448
    Illegal NgoMIV site found at 3186
    Illegal NgoMIV site found at 3641
    Illegal AgeI site found at 1450
    Illegal AgeI site found at 2716
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None